Growth and structural properties of epithelial cell cultures established from normal rat liver and chemically induced hepatomas.
نویسندگان
چکیده
Epithelial cultures established from adult rat liver and from rat hepatomas induced in vivo with aromatic amine carcinogens have been compared by light and electron microscopy and by growth properties in liquid medium and in agar. The morphology and growth patterns of all of these cultures indicate that they have characteristics of epithelial rather than fibroblast cells. The criteria generally used to score for transformation of fibroblasts were not satisfactory for distinguishing normal epithelial cells from hepatoma cells in culture. Growth in agar, however, provides a simple and objective method of scoring for transformed epithelial cells, because only the tumorigenic cells grow in agar. Since none of the normal cultures had hydrocortisone-inducible tryosine aminotransferase, we lack definitive evidence that they are derived from liver parenchymal cells. The outstanding feature in the ultrastructure of the hepatoma cells in culture was the presence of type A and C viral particles. Whereas five hepatoma cultures and a spontaneously transformed normal liver cell line were positive for these particles, five independently isolated cell cultures from normal adult rat liver were negative. Evidence is presented that the viral particles seen in hepatoma cultures are due to activation of latent viruses rather than to in vitro contamination. The possible significance of these particles in hepatocarcinogenesis is discussed.
منابع مشابه
Changes in the PPla mRNA Level and PP1 Activity of Ascites Hepatomas at Different Cell Growth Rates
It is now well established that the type la of serine/threonine protein phosphatases (PP1α) may be implicated in malignant phenotype. Although the PP1α mRNA level increased in livers at preneo-plastic stages of hepatocarinogenesis and all of examined rat ascites hepatomas, unexpectedly, dra-matically decreased in some of primary hepatomas. In order to elucidate the low level of PP1α mRNA in som...
متن کاملPhenotypic expression in spontaneously transformed cultured rat liver epithelial cells.
Five clonal cell strains of an early-passage normal rat liver epithelial cell line were transformed spontaneously using the protocol of "selective culture" condition. Twelve cell lines were established from the tumors produced after injecting these transformed cells into 1-day-old syngeneic rats. The phenotypic expressions of these spontaneously transformed tumor cell lines were studied and com...
متن کاملFilamentous forms of enveloped A particles in cell cultures from chemically induced rat hepatomas.
متن کامل
Attenuation by l-thyroxine of oxidant-induced gut epithelial damage
Objective(s): Severe injuries are often associated with tissue hypothyroidism, elevated damaging mediators in circulation, and broken gut epithelial barrier. However, the relationships between the hypothyroid state and gut epithelial damage are largely unknown. Therefore, in this study, we investigated the effects of L-thyroxine (T4) on in vitro models of intact and ...
متن کاملExpression of autophosphorylating protein kinase 500 in normal and neoplastic rat cells.
Autophosphorylating protein kinase 500 (AUT-PK 500) is a unique serine protein kinase that was originally purified and characterized from the rat adrenocortical carcinoma. A specific RIA with an assay sensitivity of 10 ng (0.02 pmol) was developed for AUT-PK 500 and applied to normal, embryonic, fetal, neonatal, immortal, and neoplastic tissues and cultured cells. As compared to normal rat tiss...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Cancer research
دوره 35 1 شماره
صفحات -
تاریخ انتشار 1975